{"id":4734,"date":"2018-05-22T08:05:57","date_gmt":"2018-05-22T08:05:57","guid":{"rendered":"http:\/\/imas12.es\/en\/?page_id=4734"},"modified":"2018-05-22T08:05:57","modified_gmt":"2018-05-22T08:05:57","slug":"confocal-microscopy-unit","status":"publish","type":"page","link":"https:\/\/imas12.es\/en\/services\/confocal-microscopy-unit\/","title":{"rendered":"Confocal Microscopy Unit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_tta_tabs style=&#8221;modern&#8221; color=&#8221;orange&#8221; active_section=&#8221;1&#8243;][vc_tta_section title=&#8221;Overview&#8221; tab_id=&#8221;1526899228973-fcfa8c36-4f6f&#8221;][vc_column_text]<\/p>\n<p>The Institute of the <em>Hospital Universitario 12 de Octubre<\/em> is supported by the Confocal Microscopy Unit of said hospital. This Unit is located in the \u201cResearch Center\u201d building of the hospital. The Managers of this Confocal Microscopy Unit are Dr. Fernando Bartolom\u00e9 and Dr. Gema Ruiz Hurtado.<\/p>\n<p>The responsibilities of the Unit\u2019s Managers are detailed below:<\/p>\n<ul>\n<li>Defining the general rules applicable to the use of the Confocal Microscopy to achieve a considerable improvement in its performance and use.<\/li>\n<li>Supervising the necessary annual external maintenance activities carried out on the equipment to ensure its correct performance, as well as all communications made with the technical services, when necessary.<\/li>\n<li>Counseling the different research groups in the development and commissioning of experiments and protocols for which the equipment must be used.<\/li>\n<li>Specific training of new users (initial training).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Service portfolio&#8221; tab_id=&#8221;1526899228995-76b479db-4838&#8243;][vc_column_text]<\/p>\n<p>The Confocal Microscopy Unit allows the researchers of the i+12 Institute to implement the following techniques and methodologies:<\/p>\n<p><strong>Confocal Microscopy<\/strong><\/p>\n<ol>\n<li style=\"list-style-type: none\">\n<ol>\n<li><em>In vivo <\/em>physiological assays.\n<ol>\n<li>Quantification of intracellular calcium (Fluo-3AM, Fluo-4AM, MagFluo-4, etc.).<\/li>\n<li>Determination of the plasma (di4ANEPS) and mitochondrial membrane (TMRM, TMRE) potential.<\/li>\n<li>Determinations of oxidative stress (DHE, GSH, Mitosox, Lipid peroxidation, etc.).<\/li>\n<li>FAD\/Redox.<\/li>\n<li>Protein colocalization and interaction (FRET).<\/li>\n<\/ol>\n<\/li>\n<li>Immunofluorescence and probe detection (apoptosis and cell proliferation, cellular and subcellular localization, diffusion assays, etc.).<\/li>\n<li>Subcellular localization with fluorescent proteins.<\/li>\n<li>Tridimensional studies (Z-Stack).<\/li>\n<li>Counterstaining: labeling of the Golgi complex (HPA lecithin, protein Grasp68), the endoplasmic reticulum (Ryanodine, Calnexin), the mitochondria (Mitotracker Red\/Green), the actin microfilaments (falotoxin) and nuclear labeling (IP, Hoechst, DAPI, etc.).<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Facilities and infrastructure&#8221; tab_id=&#8221;1526899239865-cc0bbf7f-22e8&#8243;][vc_column_text]<\/p>\n<p>To apply these techniques and methodologies, the Confocal Microscopy Unit of the i+12 Institute features the following high-tech equipment:<\/p>\n<ul>\n<li>LSM510 META ConfoCor 3 Spectral Confocal Microscope (ZEISS).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Staff and contact information&#8221; tab_id=&#8221;1526899240516-ff8290c5-e4e5&#8243;][vc_column_text]<\/p>\n<p>Gema Ruiz Hurtado.\u00a0<a href=\"mailto:gemaruiz@h12o.es\">gemaruiz@h12o.es<\/a>; Telephone No.: 91 390 80 01<\/p>\n<p>Fernando Bartolom\u00e9 Robledo.\u00a0<a href=\"mailto:fbartolome.imas12@h12o.es\">fbartolome.imas12@h12o.es<\/a>; Telephone No.: 91 390 87 65<\/p>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Rates and rules of use&#8221; tab_id=&#8221;1526899241127-28b0047c-6401&#8243;][vc_column_text]<a href=\"http:\/\/imas12.es\/wp-content\/uploads\/2018\/Servicios\/Rates_ENG\/Confocal rates 2018.pdf\" target=\"_blank\" rel=\"noopener\">Access to rates of the Confocal Microscopy Unit<\/a>.<\/p>\n<p><a href=\"http:\/\/imas12.es\/wp-content\/uploads\/2018\/Servicios\/Rates_ENG\/Rules of use - confocal.pdf\" target=\"_blank\" rel=\"noopener\">Rules of use of the Confocal Equipment<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Images&#8221; tab_id=&#8221;1526899241917-fc784cc3-3019&#8243;][vc_images_carousel images=&#8221;4745,4746,4747,4748,4749,4750,4751,4752&#8243; img_size=&#8221;large&#8221; wrap=&#8221;yes&#8221;][\/vc_tta_section][\/vc_tta_tabs][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_tta_tabs style=&#8221;modern&#8221; color=&#8221;orange&#8221; active_section=&#8221;1&#8243;][vc_tta_section title=&#8221;Overview&#8221; tab_id=&#8221;1526899228973-fcfa8c36-4f6f&#8221;][vc_column_text] The Institute of the Hospital Universitario 12 de Octubre is supported by the Confocal Microscopy Unit of said hospital. This Unit is located in the \u201cResearch Center\u201d building of the hospital. The Managers of&#8230;<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":94,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4734","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/pages\/4734","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/comments?post=4734"}],"version-history":[{"count":13,"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/pages\/4734\/revisions"}],"predecessor-version":[{"id":4790,"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/pages\/4734\/revisions\/4790"}],"up":[{"embeddable":true,"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/pages\/94"}],"wp:attachment":[{"href":"https:\/\/imas12.es\/en\/wp-json\/wp\/v2\/media?parent=4734"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}